The UniGene-based Transcriptional Profiles of Human Tissues
Released November, 13th, 2000
- The expression profiles of human tissues. To analyze the transcriptional activity of specific human tissues, the number of ESTs corresponding to each UniGene entry, found in a selected set cDNA libraries were counted, on the assumption that the larger is the number of ESTs reported per entry, the more active should be in the tissue the corresponding gene.
The relative contribution of a given gene to the tissue transcriptional activity is expressed by the ratio between the number of ESTs corresponding to that gene and the total number of ESTs corresponding to all the genes included in the data set.
This number represents the best available estimate of the abundance of each individual transcript in the given tissue mRNA population and it is referred here as "level of expression" of each gene.
- Expression of genes in other tissues. The presence of the genes in other human tissues was assessed by considering each UniGene entry belonging to the data set and by recording in which additional tissues the ESTs reported in that particular entry were found.
- Data retrieval. Data on the UniGene entries belonging to selected libraries were retrieved from UniGene (table below).
The catalogue of the genes expressed in a specific tissue, including transcripts expressed as well in other tissues, provides the best representation of its transcriptome, according to available information.
- For each tissue the catalogues of genes or ESTs reported in UniGene as belonging to the selected cDNA libraries is provided. Each entry is shown along with UniGene ID, gene description, estimated level of expression in the tissue and information on expression in additional human adult tissues.
Tissue UniGene cDNA libraries
ESTs UniGene clusters Uterus 119, 312, 732, 733, 3600 95776 11688 Ovary 576, 652, 935, 1369, 1380, 3223, 3225 30256 2741 Brain 128, 859, 857, 15, 255 66286 2473 66286 2473 Liver 155 8257 1351 Skeletal Muscle 24, 272, 500 30231 3688 Retina 177, 178, 228, 313 21054 4631
Return to GETProfiles