THE UNIGENE-BASED RETINA TRANSCRIPTIONAL PROFILE
Released January, 14th, 1999
The human retina trancriptional
profile. To analyze the transcriptional
activity of the retina human tissue, the number of ESTs corresponding to
each UniGene entry, found in the considered cDNA libraries were counted,
on the assumption that the larger is the number of retina ESTs reported
per entry, the more active should be in the tissue the corresponding gene.
This number represents the best available estimate of the abundance of each
individual transcript in the retina mRNA population and it is referred
here as "level of expression" of each gene. The relative contribution
of a given gene to the retina transcriptional activity is expressed
by the ratio between the number of ESTs corresponding to that gene
and the total number of ESTs corresponding to all the genes included
in the data set.
Expression of retina genes in other tissues. The presence of retina genes
in other human tissues was assessed by considering each UniGene entry
belonging to the data set and by recording in which additional tissues
the ESTs reported in that particular entry were found.
Data retrieval. The retrieval of UniGene entries belonging to the four
most copious not normalized nor subtracted cDNA libraries from normal
adult retina (Lib.177, Lib.178, Lib.228 and Lib.313) produced a data
set of 4,975 putative unique human genes.
The catalogue of these 4,975 genes, including both the retina-specific
gene transcripts and transcripts expressed as well in other tissues,
provides the best representation of the transcriptome of the retina,
according to available information.
The catalogue of 4,975 genes or
reported in UniGene as belonging
to cDNA libraries from retina is provided. Each entry is shown along
with UniGene ID, gene description, estimated level of expression in
retina and information on expression in additional human adult
Reference Bortoluzzi S., d'Alessi F., Danieli G.A., "A novel resource
for the study of genes expressed in the adult human retina",
Invest.Ophth.Vis.Sci. (in press)
Return to GETProfiles