THE UNIGENE-BASED HUMAN CARDIAC MUSCLE TRANSCRIPTIONAL PROFILE
Released June, 26th, 2000
- The human cardiac muscle trancriptional
To analyze the transcriptional activity of the heart human tissue, the
number of ESTs corresponding to each UniGene entry, found in the selected
cDNA libraries were counted, on the assumption that the larger is the
number of heart ESTs reported per entry, the more active should be in the
tissue the corresponding gene.
The relative contribution of a given gene to the heart transcriptional
activity is expressed by the ratio between the number of ESTs corresponding
to that gene and the total number of ESTs corresponding to all the genes
included in the data set.
This number represents the best available
estimate of the abundance of each individual transcript in the heart mRNA
population and it is referred here as "level of expression" of each gene.
- Expression of cardiac muscle genes in other tissues.
The presence of heart genes in other human tissues was assessed by
considering each UniGene entry belonging to the data set and by recording
in which additional tissues the ESTs reported in that particular entry were
- Data retrieval.
Data on the UniGene entries belonging to selected heart
libraries were retrieved from UniGene.
Two different data sets were considered:
- The 996 putative human genes represented in a group of four unormalized
and unsubtracted heart libraries (Lib.47, Lib.46, Lib251. Lib.10)
- The 2,077 putative human genes represented in library Lib.47 or Lib.46 or
Lib251 or Lib.10 or in a partially normalized library (Lib.241).
The catalogue of the 2,077 heart genes, including transcripts expressed as
well in other tissues, provides the best representation of the
transcriptome of the human heart, according to available information.
- The catalogue
of 2,077 genes or ESTs reported in UniGene as belonging to
cDNA libraries from heart is provided. Each entry is shown along with
UniGene ID, gene description, estimated level of expression in heart and
information on expression in additional human adult tissues.
For the genes belonging to both data sets, two different estimates are
given of the level of expression in percentage (Rows 3 and 4).
In addition, the genes were classified according to the strength of the
bias introduced by the presence of ESTs derived from a partially normalized library.
Some estimated levels of expression are considered as partially biased
or biased, according to the usage of some "normalized" ESTs or of
"normalized" ESTs only, when calculating the level of expression.
- Reference Bortoluzzi S., d'Alessi F., Danieli G.A., "A computational
reconstruction of the adult human heart transcriptional profile", J. Mol. Cell. Cardiol.
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